5H3G

Crystal Structure of Oryza sativa Acyl-CoA-Binding Protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9900
Spacegroup name	P 32 2 1
Unit cell lengths	59.680, 59.680, 60.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.357 - 1.600
R-factor	0.1927
Rwork	0.191
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fj9
RMSD bond length	0.005
RMSD bond angle	0.771
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.400	39.400	1.600
High resolution limit [Å]	1.500	10.000	1.500
Rmerge	0.091	0.057	2.642
Rmeas	0.094	0.060	2.704
Total number of observations	429466
Number of reflections	20012	64	3342
<I/σ(I)>	20.32	38.74	2.1
Completeness [%]	97.8	74.4	94.5
Redundancy	21.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	295	0.1M Citric acid, 2.6M NaCl