5H3C

Crystal structure of Arabidopsis SNC1 TIR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	110
Detector technology	CCD
Collection date	2016-06-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97922
Spacegroup name	P 43 21 2
Unit cell lengths	81.534, 81.534, 125.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.331 - 2.596
R-factor	0.2249
Rwork	0.219
R-free	0.27790
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	13649
<I/σ(I)>	16.7
Completeness [%]	99.2
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	0.3M Ammonium acetate, 35% PEG 3350, 0.1M HEPES pH 7.5, 6% Glycerol