5H33
Structural basis for dimerization of the death effector domains of Caspase-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.420, 50.940, 90.444 |
| Unit cell angles | 90.00, 105.24, 90.00 |
Refinement procedure
| Resolution | 43.632 - 3.600 |
| R-factor | 0.283410396923 |
| Rwork | 0.280 |
| R-free | 0.31256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zbw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.644 |
| Data reduction software | CrystalClear (1.4) |
| Data scaling software | d*TREK (9.7) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.440 |
| High resolution limit [Å] | 3.600 |
| Number of reflections | 5874 |
| <I/σ(I)> | 4.4 |
| Completeness [%] | 99.6 |
| Redundancy | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 50mM sodium chloride, 100mM Tris pH 8.5, 22.5% PEG3350 |
