5H2W

Crystal structure of the karyopherin Kap60p bound to the SUMO protease Ulp1p (150-340)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	52.610, 63.540, 80.910
Unit cell angles	106.09, 107.72, 90.50

Refinement procedure

Resolution	27.028 - 2.500
R-factor	0.2152
Rwork	0.213
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c1t
RMSD bond length	0.003
RMSD bond angle	0.822
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.8)
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.370	30.370	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.050	0.025	0.163
Rmeas	0.070
Rpim	0.050
Total number of observations	47966
Number of reflections	29881
<I/σ(I)>	5.9
Completeness [%]	90.5	91.3	91
Redundancy	1.6	1.9	1.7
CC(1/2)	0.995	0.996	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1M MES, 0.1M calcium acetate, 12% PEG8000