5H2D
Crystal structure of Osh1 ORD domain in complex with ergosterol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.129, 47.207, 73.000 |
| Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
| Resolution | 37.280 - 1.600 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4inq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.065 | 0.306 |
| Number of reflections | 54668 | |
| <I/σ(I)> | 26.1 | 5.3 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | Bicine-HCl pH 9.0, 20% PEG 3350, 0.2M MgSO4 |
