5H2C

Crystal structure of Saccharomyces cerevisiae Osh1 ANK - Nvj1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	59.896, 67.748, 107.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.726 - 3.508
R-factor	0.1878
Rwork	0.184
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.879
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.182	0.415
Number of reflections	5793
<I/σ(I)>	17	7.3
Completeness [%]	99.7	100
Redundancy	7.4	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.1M Citrate-NaOH pH 5.0, 15% PEG 20000, 0.2M KNO3