5H2B

Structure of a novel antibody G196

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	38.647, 82.267, 127.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.134 - 2.001
R-factor	0.2147
Rwork	0.211
R-free	0.28070
RMSD bond length	0.008
RMSD bond angle	1.144
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	26685
<I/σ(I)>	18.7
Completeness [%]	94.3
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293.2	0.1M sodium citrate, 20% PEG 20000