5H1M

Crystal structure of WD40 repeat domains of Gemin5 in complex with M7G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 1 21 1
Unit cell lengths	60.534, 120.907, 61.257
Unit cell angles	90.00, 117.42, 90.00

Refinement procedure

Resolution	29.341 - 2.492
R-factor	0.1849
Rwork	0.182
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h1j
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.070	0.040	0.609
Rmeas	0.084	0.047	0.723
Rpim	0.045	0.026	0.387
Total number of observations	89686
Number of reflections	26965
<I/σ(I)>	11.2
Completeness [%]	99.1	96.7	99.6
Redundancy	3.3	3.4	3.3
CC(1/2)		0.996	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M HEPES (pH 7.5), 14% PEG 8000, 25mM MgCl2