5H17
EED in complex with PRC2 allosteric inhibitor EED210
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979112 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.560, 92.280, 180.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.1918 |
| Rwork | 0.189 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qxv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.281 |
| High resolution limit [Å] | 2.200 | 2.274 |
| Rmerge | 0.103 | |
| Number of reflections | 21839 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris, 16% PEG 8000,10 mM beta-Nicotinamide mononucleotide |
