5H12

Crystal structure of Deep Vent DNA Polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	77.005, 112.026, 111.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.297 - 2.502
R-factor	0.2175
Rwork	0.214
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.557
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2299)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	33815
<I/σ(I)>	13.5
Completeness [%]	91.2
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium citrate tribasic dehydrate (pH 5.6), 0.1 M lithium sulfate monohydrate, 12 % (w/v) polyethylene glycol 6000