5H0T
Crystal structure of proliferating cell nuclear antigen from Leishmania donovani at 2.73 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.800, 149.574, 169.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 112.080 - 2.730 |
| R-factor | 0.19783 |
| Rwork | 0.197 |
| R-free | 0.23915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5B7O |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.080 | 2.780 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Number of reflections | 89366 | |
| <I/σ(I)> | 12 | 1.45 |
| Completeness [%] | 90.7 | 80 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG 3350, sodium malonate |
