5H0O
Crystal structure of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease with manganese ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-28 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.847, 66.847, 51.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.856 - 1.530 |
| R-factor | 0.1421 |
| Rwork | 0.141 |
| R-free | 0.17360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h0m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.065 | |
| Number of reflections | 19106 | |
| <I/σ(I)> | 21.6 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES, 0.2M sodium chloride, pH 7.5, 25% (w/v) polyethylene glycol 4000 |
