5GZT

Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	114.339, 123.486, 130.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.100
R-factor	0.17762
Rwork	0.175
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1itx
RMSD bond length	0.021
RMSD bond angle	1.985
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.367
Number of reflections	108304
<I/σ(I)>	50.1	4.42
Completeness [%]	99.9	100
Redundancy	5.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	5.5	297	(NH4)2HPO4, Na-citrate