5GZO

Structure of neutralizing antibody bound to Zika envelope protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	128.668, 97.504, 165.753
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	44.680 - 2.755
R-factor	0.2334
Rwork	0.231
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhm 5jhl
RMSD bond length	0.005
RMSD bond angle	1.074
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.760	2.760
Number of reflections	53031
<I/σ(I)>	9.903
Completeness [%]	99.9
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.12 M Alcohols, 0.1 M Buffer System 2, 50%(v/v) Precipitant Mix 2