5GZM
Cyclodeaminase_PA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.779, 74.131, 95.761 |
| Unit cell angles | 90.00, 132.84, 90.00 |
Refinement procedure
| Resolution | 37.790 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20200 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.805 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.061 | 0.490 |
| Number of reflections | 63049 | |
| <I/σ(I)> | 26.02 | 2.01 |
| Completeness [%] | 96.5 | |
| Redundancy | 6.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2% (v/v) Polyethylene glycol 400, 0.1M HEPES |
