5GZL
Cyclodeaminase_PA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.094, 92.825, 117.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.000 |
| R-factor | 0.13955 |
| Rwork | 0.137 |
| R-free | 0.18498 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.741 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.491 |
| Number of reflections | 47575 | |
| <I/σ(I)> | 18.68 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2%(v/v) polyethylene glycol 400, 0.1M HEPES |
