5GZJ
Cyclodeaminase_PA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-08-21 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.604, 93.250, 118.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.630 - 1.870 |
| R-factor | 0.15494 |
| Rwork | 0.153 |
| R-free | 0.19396 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.071 | 0.481 |
| Number of reflections | 59165 | |
| <I/σ(I)> | 19.48 | 3.31 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2%(v/v) polyethylene glycol 400, 0.1M HEPES |
