5GYZ

luciferase AMP/7-cy-L complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 41
Unit cell lengths	73.443, 73.443, 96.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.200 - 2.400
R-factor	0.176
Rwork	0.173
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ba3
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	19770
<I/σ(I)>	47.1
Completeness [%]	98.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	Sodium malonate, PEG3350