5GYR
Tetrameric Allochromatium vinosum cytochrome c'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.949, 48.620, 130.550 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 41.400 - 1.600 |
| R-factor | 0.20546 |
| Rwork | 0.204 |
| R-free | 0.23228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.331 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.400 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | 0.325 |
| Number of reflections | 135869 | |
| <I/σ(I)> | 7.9 | 2.9 |
| Completeness [%] | 98.6 | 97.2 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.1mM sodium acetate, 0.2mM ammonium acetate, 30% (w/v) polyethylene glycol 3350 |
