5GYL
Structure of Cicer arietinum 11S gloubulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 97.370, 100.610, 131.124 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 41.000 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.689 |
| Data reduction software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.650 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 63945 |
| <I/σ(I)> | 2 |
| Completeness [%] | 99.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 5% PEG 3350, 0.1M MES pH7.0 |
