5GYK

Crystal Structure of Mdm12-deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97957
Spacegroup name	P 21 21 21
Unit cell lengths	109.239, 148.243, 212.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.451 - 3.596
R-factor	0.222
Rwork	0.220
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gyd
RMSD bond length	0.007
RMSD bond angle	1.369
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.596	3.600
Rmerge	0.142
Number of reflections	40722
<I/σ(I)>	11.1
Completeness [%]	99.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium sulfate, Tris-HCl (pH 8.5), Lithium sulfate