5GYI
Crystal structure of ENZbleach xylanase V176C+E220C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-25 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 42.664, 70.146, 86.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.970 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gv1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 4.240 | 1.970 |
| Rmerge | 0.102 | 0.034 | 0.397 |
| Number of reflections | 18798 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.4 | 99.9 | 97.8 |
| Redundancy | 3.6 | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 298 | PEG 8000, 0.2 M NaCl, 0.1 M Phosphate-citrate |
