5GYF
Crystal structure of ENZbleach xylanase T28C+T60C+L59F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-20 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 42.132, 70.022, 83.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.940 |
| R-factor | 0.2047 |
| Rwork | 0.202 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gv1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.398 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.010 | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 4.160 | 1.930 |
| Rmerge | 0.063 | 0.040 | 0.221 |
| Number of reflections | 18671 | ||
| <I/σ(I)> | 15.8 | ||
| Completeness [%] | 99.8 | 99.9 | 99.2 |
| Redundancy | 7.5 | 9.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 298 | PEG MME 2000, 0.1 M MES monohydrate |
