5GY9
Crystal structure of ENZbleach xylanase A74C+G84C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-18 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.594, 83.506, 79.526 |
| Unit cell angles | 90.00, 96.52, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.940 |
| R-factor | 0.1887 |
| Rwork | 0.186 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gv1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 4.180 | 1.940 |
| Rmerge | 0.077 | 0.037 | 0.268 |
| Number of reflections | 41799 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 99.6 | 99.8 | 96.9 |
| Redundancy | 3.8 | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 298 | PEG 3350, 0.2 M NH4Cl |
