5GXV
Crystal structure of PigG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2015-06-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.131, 55.131, 262.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.745 - 2.100 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23170 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.800 | |
| Number of reflections | 28301 | |
| <I/σ(I)> | 8.3 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.3 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MgCl2,Tris-HCl, pH 8.5, PEG 4000 |
