5GXU

Cystal structure of Arabidopsis ATR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	55.333, 61.821, 88.331
Unit cell angles	97.84, 100.51, 90.27

Refinement procedure

Resolution	30.608 - 2.300
R-factor	0.2021
Rwork	0.200
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amo
RMSD bond length	0.006
RMSD bond angle	1.013
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.452
Number of reflections	48501
<I/σ(I)>	10.9	2.03
Completeness [%]	96.7	97.3
Redundancy	1.9	2
CC(1/2)		0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	21% (w/v)5000 MME, 0.1M MES