5GXQ
The crystal structure of the nucleosome containing H3.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.026, 109.764, 181.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.129 - 2.850 |
R-factor | 0.2213 |
Rwork | 0.219 |
R-free | 0.26180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.010 |
RMSD bond angle | 1.147 |
Data scaling software | HKL-2000 (0.98.704k) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
Rmerge | 0.084 | 0.048 | 0.464 |
Total number of observations | 297098 | ||
Number of reflections | 50454 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 99.6 | 96.5 | 100 |
Redundancy | 5.9 | 5.4 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |