5GXH

The structure of the Gemin5 WD40 domain with AAUUUUUG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791829
Spacegroup name	P 1 21 1
Unit cell lengths	58.138, 124.459, 60.808
Unit cell angles	90.00, 116.96, 90.00

Refinement procedure

Resolution	47.840 - 1.800
R-factor	0.16348
Rwork	0.162
R-free	0.19656
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ow8 2hes 3dm0
RMSD bond length	0.020
RMSD bond angle	1.860
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	71182
<I/σ(I)>	21.2	2.3
Completeness [%]	100.0	100
Redundancy	7.4	7.5
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M sodium citrate, 0.2 M ammonium acetate, 15% PEG 4000