5GX8
Crystal structure of solute-binding protein related to glycosaminoglycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.674, 142.050, 73.676 |
| Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
| Resolution | 39.364 - 1.778 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gub |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.246 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.057 | 0.407 |
| Number of reflections | 139193 | |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 98.6 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M tri-sodium citrate dehydrate (pH 5.6), 2M ammonium sulfate |
