Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5GX4

Luciferin-regenerating enzyme collected with serial synchrotron rotational crystallography with accumulated dose of 14 MGy (12th measurement)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyPIXEL
Collection date2016-05-30
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.9839
Spacegroup nameP 21 21 21
Unit cell lengths47.510, 76.962, 84.433
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution42.216 - 1.600
R-factor0.1802
Rwork0.180
R-free0.19210
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)5gtq
RMSD bond length0.006
RMSD bond angle0.853
Phasing softwarePHENIX (1.10.1_2155)
Refinement softwarePHENIX (1.10.1_2155)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0001.664
High resolution limit [Å]1.6003.3281.600
Total number of observations2851956
Number of reflections79032
<I/σ(I)>6.42273215.431.38
Completeness [%]100.0100100
Redundancy36.086137.832.5
CC(1/2)0.9920.9910.466
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1BATCH MODE7.5293150 uL of purified LRE solution (30 mg/ml LRE, 10 mM HEPES(pH7.5), 100 mM NaCl, 10 %(v/v) glycerol) was mixed with 240 uL of precipitant solution (31.3 %(w/v) PEG3350, 0.1 M HEPES(pH7.5), 10 %(v/v) MPD, 0.2 M MgCl2) and 15 uL of seed-crystal solution (31.3 %(w/v) PEG3350, 0.1 M HEPES(pH7.5), 10 %(v/v) MPD, 0.2 M MgCl2, 0.1 M NaCl, 5 %(v/v) glycerol)

229380

PDB entries from 2024-12-25

PDB statisticsPDBj update infoContact PDBjnumon