5GVT

Crystal structures of the serine protease domain of murine plasma kallikrein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	72.231, 85.556, 109.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.040 - 2.610
R-factor	0.221
Rwork	0.219
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2any
RMSD bond length	0.015
RMSD bond angle	1.690
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	21266
<I/σ(I)>	13.7
Completeness [%]	98.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	PEG 3350 25%, 0.1M Tris-HCl, 0.1M NaCl