5GVH

Structure of FabK from Thermotoga maritima

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-21
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9789, 0.9792, 0.9814
Spacegroup name	C 1 2 1
Unit cell lengths	65.611, 77.107, 59.085
Unit cell angles	90.00, 99.94, 90.00

Refinement procedure

Resolution	40.453 - 2.294
R-factor	0.197
Rwork	0.193
R-free	0.23030
Structure solution method	MAD
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.500	2.380
High resolution limit [Å]	2.290	2.300
Number of reflections	22681
<I/σ(I)>	34.5
Completeness [%]	94.5
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Bicine, pH 9.0, 1.5 M LiCl, 23% PEG 6000
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Bicine, pH 9.0, 1.5 M LiCl, 23% PEG 6000
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Bicine, pH 9.0, 1.5 M LiCl, 23% PEG 6000