5GV8
Structure of NADH-cytochrome b5 reductase refined with the multipolar atomic model at 0.78A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 40 |
| Detector technology | CCD |
| Collection date | 2011-02-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.480, 72.108, 84.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.900 - 0.780 |
| Rwork | 0.122 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ndh |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 0.790 |
| High resolution limit [Å] | 0.780 | 0.780 |
| Number of reflections | 332946 | |
| <I/σ(I)> | 28.8 | 1.1 |
| Completeness [%] | 99.1 | 89.5 |
| Redundancy | 6.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG 4000, DTT, K-phos buffer |
