5GV3

Crystal structure of the membrane-distal domain of mouse lysosome-associated membrane protein 2 (LAMP-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.2
Spacegroup name	C 2 2 21
Unit cell lengths	59.610, 103.930, 129.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.488 - 2.096
R-factor	0.272
Rwork	0.269
R-free	0.30750
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.149
High resolution limit [Å]	2.096	2.096
Number of reflections	23657
<I/σ(I)>	16.31
Completeness [%]	98.7
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50mM zinc acetate, 20% (v/v) polyethylene glycol 3500