5GV1
Crystal structure of ENZbleach xylanase wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.716, 62.519, 61.247 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 29.910 - 1.500 |
| R-factor | 0.1902 |
| Rwork | 0.190 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data scaling software | d*TREK (7.1SSI) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 29.910 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.045 | 0.035 | 0.110 |
| Number of reflections | 38524 | ||
| <I/σ(I)> | 11.2 | 20.8 | 4.5 |
| Completeness [%] | 91.0 | 91.8 | 85.8 |
| Redundancy | 2.27 | 2.28 | 2.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 298 | PEG 1500, 0.1 M Bis-Tris |
