5GV0

Crystal structure of the membrane-proximal domain of mouse lysosome-associated membrane protein 1 (LAMP-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	31.596, 50.047, 95.144
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.480 - 1.500
R-factor	0.1761
Rwork	0.174
R-free	0.19630
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.059	0.037	0.148
Number of reflections	24809
<I/σ(I)>	16.4
Completeness [%]	99.3	95.6	94.1
Redundancy	6.6	6.3	4.5
CC(1/2)		0.999	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2M ammonium sulphate, 0.1M sodium HEPES (pH 7.5), 2% (v/v) polyethylene glycol 400