5GUB
Crystal structure of solute-binding protein complexed with sulfate group-free unsaturated chondroitin disaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.914, 112.213, 166.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.569 - 1.780 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y3n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.065 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.058 | 0.310 |
Number of reflections | 143362 | |
<I/σ(I)> | 21.8 | |
Completeness [%] | 99.2 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium chloride, 0.1M MES (pH 6.0), 20% PEG 6000 |