5GUA
Structure of biotin carboxyl carrier protein from pyrococcus horikoshi OT3 (delta N79) A138Y mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 39.063, 39.063, 76.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.800 - 1.500 |
R-factor | 0.1861 |
Rwork | 0.185 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2evb |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.800 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.044 | 0.102 |
Number of reflections | 11290 | |
<I/σ(I)> | 41.2 | 16 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 9.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.5M sodium chloride, 0.2M lithium sulfate, 0.1M acetate-NaOH |