5GU4
rRNA N-glycosylase RTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.450, 59.880, 67.495 |
| Unit cell angles | 90.00, 99.89, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.550 |
| R-factor | 0.2195 |
| Rwork | 0.218 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3px8 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.046 | 0.035 | 0.339 |
| Rmeas | 0.054 | 0.042 | 0.397 |
| Rpim | 0.028 | 0.022 | 0.205 |
| Total number of observations | 272881 | ||
| Number of reflections | 75182 | ||
| <I/σ(I)> | 17.2 | ||
| Completeness [%] | 97.8 | 96.5 | 96.4 |
| Redundancy | 3.6 | 3.4 | 3.6 |
| CC(1/2) | 0.997 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2.8 M sodium acetate, tetrahydrate pH7.0 30%-35% glucose |
