Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.527, 93.284, 145.472 |
| Unit cell angles | 90.00, 98.05, 90.00 |
Refinement procedure
| Resolution | 49.570 - 2.600 |
| R-factor | 0.18548 |
| Rwork | 0.182 |
| R-free | 0.25095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fr8 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.780 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.046 | 0.075 |
| Number of reflections | 67464 | |
| <I/σ(I)> | 69.7 | |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 0.15 M DL-malic acid pH 7.0, 18% PEG 3350 |
