5GT5
Structural basis of the specific activity and thermostability of pectate lyase (pelN) from Paenibacillus sp. 0602
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-08 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.552, 55.584, 83.182 |
| Unit cell angles | 74.26, 84.40, 73.51 |
Refinement procedure
| Resolution | 34.431 - 1.449 |
| R-factor | 0.1507 |
| Rwork | 0.149 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zsc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.440 |
| Number of reflections | 629583 |
| <I/σ(I)> | 14.6 |
| Completeness [%] | 99.8 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 10% PEG 3000, 100mM Na2HPO4 |
