5GT0

Crystal structure of nucleosome complex with human testis-specific histone variants, Th2a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	99.788, 108.966, 170.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.800 - 2.820
R-factor	0.214
Rwork	0.210
R-free	0.28000
RMSD bond length	0.011
RMSD bond angle	1.338
Data scaling software	HKL-2000 (Scala)
Phasing software	CNX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge		0.597
Number of reflections	45145
<I/σ(I)>	16.2
Completeness [%]	99.7	99.7
Redundancy	6.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	60-70mM KCl, 70-90mM MnCl2, 24% MPD