5GSX
Mouse MHC class I H-2Kd with a MERS-CoV-derived peptide 142-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.623, 68.474, 145.075 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 47.008 - 2.500 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.24190 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 123285 |
| <I/σ(I)> | 15 |
| Completeness [%] | 93.6 |
| Redundancy | 2.345 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6000 |
