5GSR
Mouse MHC class I H-2Kd with a MERS-CoV-derived peptide I5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.353, 79.658, 122.706 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 31.829 - 2.198 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23090 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.773 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.198 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 94.3 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium HEPES 7.5, 30 % v/v 2-Propanol |
