5GSC

Crystal structure of a class C beta lactamase of Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	71.511, 90.200, 113.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.161 - 1.953
R-factor	0.1862
Rwork	0.184
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zku
RMSD bond length	0.008
RMSD bond angle	1.110
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Number of reflections	53385
<I/σ(I)>	58.4
Completeness [%]	98.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	295	0.1M Cadmium chloride, 0.1M Na-Cacodylate pH4.6, 30% PEG 400