5GRQ

Crystal Structure of DHB domain of Daxx in complex with an ATRX peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2015-09-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	53.428, 56.217, 102.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.336 - 1.584
R-factor	0.1585
Rwork	0.158
R-free	0.17890
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SHELXDE
Refinement software	PHENIX ((dev_2689: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.580	4.290	1.580
Rmerge	0.076	0.040	0.515
Number of reflections	80904
<I/σ(I)>	6
Completeness [%]	99.5	99.8	90.5
Redundancy	12.8	12.4	10.4
CC(1/2)		0.998	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	100mM MES 6.0, 100mM zinc acetate, 13% ethanol