5GRQ
Crystal Structure of DHB domain of Daxx in complex with an ATRX peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2015-09-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.428, 56.217, 102.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.336 - 1.584 |
| R-factor | 0.1585 |
| Rwork | 0.158 |
| R-free | 0.17890 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.786 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 4.290 | 1.580 |
| Rmerge | 0.076 | 0.040 | 0.515 |
| Number of reflections | 80904 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 99.5 | 99.8 | 90.5 |
| Redundancy | 12.8 | 12.4 | 10.4 |
| CC(1/2) | 0.998 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 100mM MES 6.0, 100mM zinc acetate, 13% ethanol |
