5GRB
Crystal structure of 2C helicase from enterovirus 71 (EV71) bound with ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977760 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.668, 54.912, 163.097 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 45.548 - 2.803 |
| R-factor | 0.2475 |
| Rwork | 0.245 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gq1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.395 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.548 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.700 | |
| Number of reflections | 59800 | |
| <I/σ(I)> | 10.88 | 1.06 |
| Completeness [%] | 95.3 | 88.8 |
| Redundancy | 2.26 | 1.85 |
| CC(1/2) | 0.998 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES, 10% PEG6000, 5% MPD and 0.5% w/v polyvinylpyrrolidone K15 |
