5GQV
Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.126, 134.126, 184.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.420 - 3.000 |
R-factor | 0.1658 |
Rwork | 0.163 |
R-free | 0.22379 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gqu |
RMSD bond length | 0.014 |
RMSD bond angle | 1.890 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.128 | 0.322 |
Number of reflections | 34353 | |
<I/σ(I)> | 40.17 | 15.12 |
Completeness [%] | 99.8 | 100 |
Redundancy | 13.9 | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | magnesium chloride, ethanol, HEPES-NaOH |