5GQJ
Crystal structure of Cypovirus Polyhedra mutant with deletion of Ser193 and Ala194
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.060, 103.060, 103.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.070 - 1.500 |
R-factor | 0.16044 |
Rwork | 0.156 |
R-free | 0.20227 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oh6 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.022 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 29249 | |
<I/σ(I)> | 7.99 | |
Completeness [%] | 100.0 | |
Redundancy | 23.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | IN CELL | 300 | In vivo crystallization |