5GQG

Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.103, 144.061, 144.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.000 - 2.700
R-factor	0.19338
Rwork	0.190
R-free	0.25260
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.616
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.136	0.624
Number of reflections	37608
<I/σ(I)>	16.3	2.6
Completeness [%]	99.8	100
Redundancy	7.3	7.3
CC(1/2)		0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	0.1 M ammonium fluoride, 0.1 M MES-NaOH, 20-21% (v/v) PEG3350, 5% MPD